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Appl Environ Microbiol. 1990 June; 56(6): 1518-1522
Copyright © 1990, American Society for Microbiology. All Rights Reserved.

Fractionation of the ß-Linked Glucans of Bradyrhizobium japonicum and Their Response to Osmotic Potential

¹ Nitrogen Fixation and Soybean Genetics Laboratory and Horticultural Crops Quality Laboratory,² Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705

ABSTRACT

Bradyrhizobium japonicum USDA 110 synthesized both extracellular and periplasmic polysaccharides when grown on mannitol minimal medium. The extracellular polysaccharides were separated into a high-molecular-weight acidic capsular extracellular polysaccharide fraction (90% of total hexose) and three lower-molecular-weight glucan fractions by liquid chromatography. Periplasmic glucans, extracted from washed cells with 1% trichloroacetic acid, gave a similar pattern on liquid chromatography. Linkage analysis of the major periplasmic glucan fractions demonstrated mainly 6-linked glucose (63 to 68%), along with some 3,6- (8 to 18%), 3- (9 to 11%), and terminal (7 to 8%) linkages. The glucose residues were ß-linked as shown by ¹H-nuclear magnetic resonance analysis. Glucan synthesis by B. japonicum cells grown on mannitol medium with 0 to 350 mM fructose as osmolyte was measured. Fructose at 150 mM or higher inhibited synthesis of periplasmic and extracellular 3- and 6-linked glucans but had no effect on the synthesis of capsular acidic extracellular polysaccharides.

^* Corresponding author.

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