This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grund, E Articles by Eichenlaub, R Search for Related Content PubMed PubMed Citation Articles by Grund, E Articles by Eichenlaub, R Agricola Articles by Grund, E Articles by Eichenlaub, R

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1990 May; 56(5): 1459-1464

Catabolism of benzoate and monohydroxylated benzoates by Amycolatopsis and Streptomyces spp.

Fakultät für Biologie, Lehrstuhl für Gentechnologie/Mikrobiologie, Universität Bielefeld, Federal Republic of Germany.

ABSTRACT

Eight actinomycetes of the genera Amycolatopsis and Streptomyces were tested for the degradation of aromatic compounds by growth in a liquid medium containing benzoate, monohydroxylated benzoates, or quinate as the principal carbon source. Benzoate was converted to catechol. The key intermediate in the degradation of salicylate was either catechol or gentisate, while m-hydroxybenzoate was metabolized via gentisate or protocatechuate. p-Hydroxybenzoate and quinate were converted to protocatechuate. Catechol, gentisate, and protocatechuate were cleaved by catechol 1,2-dioxygenase, gentisate 1,2-dioxygenase, and protocatechuate 3,4-dioxygenase, respectively. The requirement for glutathione in the gentisate pathway was dependent on the substrate and the particular strain. The conversion of p-hydroxybenzoate to protocatechuate by p-hydroxybenzoate hydroxylase was gratuitously induced by all substrates that were metabolized via protocatechuate as an intermediate, while protocatechuate 3,4-dioxygenase was gratuitously induced by benzoate and salicylate in two Amycolatopsis strains.

This article has been cited by other articles:

• Guo, Y., Zheng, W., Rong, X., Huang, Y. (2008). A multilocus phylogeny of the Streptomyces griseus 16S rRNA gene clade: use of multilocus sequence analysis for streptomycete systematics. Int. J. Syst. Evol. Microbiol. 58: 149-159 [Abstract] [Full Text]  
• Majumdar, S., Prabhagaran, S. R., Shivaji, S., Lal, R. (2006). Reclassification of Amycolatopsis orientalis DSM 43387 as Amycolatopsis benzoatilytica sp. nov.. Int. J. Syst. Evol. Microbiol. 56: 199-204 [Abstract] [Full Text]  
• Ishiyama, D., Vujaklija, D., Davies, J. (2004). Novel Pathway of Salicylate Degradation by Streptomyces sp. Strain WA46. Appl. Environ. Microbiol. 70: 1297-1306 [Abstract] [Full Text]  
• Fairley, D. J., Boyd, D. R., Sharma, N. D., Allen, C. C. R., Morgan, P., Larkin, M. J. (2002). Aerobic Metabolism of 4-Hydroxybenzoic Acid in Archaea via an Unusual Pathway Involving an Intramolecular Migration (NIH Shift). Appl. Environ. Microbiol. 68: 6246-6255 [Abstract] [Full Text]  
• Iwagami, S. G., Yang, K., Davies, J. (2000). Characterization of the Protocatechuic Acid Catabolic Gene Cluster from Streptomyces sp. Strain 2065. Appl. Environ. Microbiol. 66: 1499-1508 [Abstract] [Full Text]  
• Gerischer, U., Segura, A., Ornston, L. N. (1998). PcaU, a Transcriptional Activator of Genes for Protocatechuate Utilization in Acinetobacter. J. Bacteriol. 180: 1512-1524 [Abstract] [Full Text]