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Appl Environ Microbiol. 1990 May; 56(5): 1378-1385

Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxins.

Plant Genetic Systems, Rijksuniversiteit Gent, Belgium.

ABSTRACT

To investigate the biochemical basis of the differences in the insecticidal spectrum of Bacillus thuringiensis insecticidal crystal proteins (ICPs), we performed membrane binding and toxicity assays with three different ICPs and three lepidopteran species. The three ICPs have different toxicity patterns in the three selected target species. Binding studies with these 125I-labeled ICPs revealed high-affinity saturable binding to brush border membrane vesicles of the sensitive species. ICPs with no toxicity against a given species did not bind saturably to vesicles of that species. Together with previous data that showed a correlation between toxicity and ICP binding, our data support the statement that differences in midgut ICP receptors are a major determinant of differences in the insecticidal spectrum of the entire lepidopteran-specific ICP family. Receptor site heterogeneity in the insect midgut occurs frequently and results in sensitivity to more than one type of ICP.

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• Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zeigler, D. R., Dean, D. H. (1998). Bacillus thuringiensis and Its Pesticidal Crystal Proteins. Microbiol. Mol. Biol. Rev. 62: 775-806 [Abstract] [Full Text]  
• Kasman, L. M., Lukowiak, A. A., Garczynski, S. F., McNall, R. J., Youngman, P., Adang, M. J. (1998). Phage Display of a Biologically Active Bacillus thuringiensis Toxin. Appl. Environ. Microbiol. 64: 2995-3003 [Abstract] [Full Text]  
• Keeton, T. P., Francis, B. R., Maaty, W. S. A., Bulla, L. A. Jr. (1998). Effects of Midgut-Protein-Preparative and Ligand Binding Procedures on the Toxin Binding Characteristics of BT-R1, a Common High-Affinity Receptor in Manduca sexta for Cry1A Bacillus thuringiensis Toxins. Appl. Environ. Microbiol. 64: 2158-2165 [Abstract] [Full Text]  
• Escriche, B., De Decker, N., Van Rie, J., Jansens, S., Van Kerkhove, E. (1998). Changes in Permeability of Brush Border Membrane Vesicles from Spodoptera littoralis Midgut Induced by Insecticidal Crystal Proteins from Bacillus thuringiensis. Appl. Environ. Microbiol. 64: 1563-1565 [Abstract] [Full Text]  
• Lally, E. T., Kieba, I. R., Sato, A., Green, C. L., Rosenbloom, J., Korostoff, J., Wang, J. F., Shenker, B. J., Ortlepp, S., Robinson, M. K., Billings, P. C. (1997). RTX Toxins Recognize a beta 2 Integrin on the Surface of Human Target Cells. J. Biol. Chem. 272: 30463-30469 [Abstract] [Full Text]  
• Tabashnik, B. E., Liu, Y.-B., Malvar, T., Heckel, D. G., Masson, L., Ballester, V., Granero, F., Mensua, J. L., Ferre, J. (1997). Global variation in the genetic and biochemical basis of diamondback moth resistance to thuringiensis. Proc. Natl. Acad. Sci. USA 94: 12780-12785 [Abstract] [Full Text]  
• Carroll, J, Wolfersberger, M., Ellar, D. (1997). The Bacillus thuringiensis Cry1Ac toxin-induced permeability change in Manduca sexta midgut brush border membrane vesicles proceeds by more than one mechanism. J. Cell Sci. 110: 3099-3104 [Abstract]  
• Liang, Y., Patel, S. S., Dean, D. H. (1995). Irreversible Binding Kinetics of Bacillus thuringiensis CryIA [IMAGE]-Endotoxins to Gypsy Moth Brush Border Membrane Vesicles Is Directly Correlated to Toxicity. J. Biol. Chem. 270: 24719-24724 [Abstract] [Full Text]  
• Masson, L., Lu, Y.-j., Mazza, A., Brousseau, R., Adang, M. J. (1995). The CryIA(c) Receptor Purified from Manduca sexta Displays Multiple Specificities. J. Biol. Chem. 270: 20309-20315 [Abstract] [Full Text]  
• Vadlamudi, R. K., Weber, E., Ji, I., Ji, T. H., Bulla, L. A. Jr. (1995). Cloning and Expression of a Receptor for an Insecticidal Toxin of Bacillus thuringiensis. J. Biol. Chem. 270: 5490-5494 [Abstract] [Full Text]  
• McGaughey, W. H., Whalon, M. E. (1992). Managing Insect Resistance to Bacillus thuringiensis Toxins. Science 258: 1451-1455 [Abstract]  
• Coux, F., Vachon, V., Rang, C., Moozar, K., Masson, L., Royer, M., Bes, M., Rivest, S., Brousseau, R., Schwartz, J.-L., Laprade, R., Frutos, R. (2001). Role of Interdomain Salt Bridges in the Pore-forming Ability of the Bacillus thuringiensis Toxins Cry1Aa and Cry1Ac. J. Biol. Chem. 276: 35546-35551 [Abstract] [Full Text]