This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, L Articles by Forsberg, C W Search for Related Content PubMed PubMed Citation Articles by Huang, L Articles by Forsberg, C W Agricola Articles by Huang, L Articles by Forsberg, C W

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1990 May; 56(5): 1221-1228

Cellulose digestion and cellulase regulation and distribution in Fibrobacter succinogenes subsp. succinogenes S85.

Department of Microbiology, University of Guelph, Ontario, Canada.

ABSTRACT

Fibrobacter succinogenes subsp. succinogenes S85 initiated growth on microcrystalline cellulose without a lag whether inoculated from a glucose, cellobiose, or cellulose culture. During growth on cellulose, there was no accumulation of soluble carbohydrate. When the growth medium contained either glucose or cellobiose in combination with microcrystalline cellulose, there was a lag in cellulose digestion until all of the soluble sugar had been utilized, suggesting an end product feedback mechanism that affects cellulose digestion. Cl-stimulated cellobiosidase and periplasmic cellodextrinase were produced under all growth conditions tested, indicating constitutive synthesis. Both cellobiosidases were cell associated until the stationary phase of growth, whereas proteins antigenically related to the Cl-stimulated cellobiosidase and a proportion of the endoglucanase were released into the extracellular culture fluid during growth, irrespective of the substrate. Immunoelectron microscopy of cells with a polyclonal antibody to Cl-stimulated cellobiosidase as the primary antibody and 10-nm-diameter gold particles conjugated to goat anti-rabbit antibodies as the second antibody revealed protrusions of the outer surface which were selectively labeled with gold, suggesting that Cl-stimulated cellobiosidase was located on the protrusions. These data support the contention that the protrusions have a role in cellulose hydrolysis; however, this interpretation is complicated by reactivity of the antibodies with a large number of other proteins that possess related antigenic epitopes.

This article has been cited by other articles:

• Jun, H.-S., Qi, M., Gong, J., Egbosimba, E. E., Forsberg, C. W. (2007). Outer Membrane Proteins of Fibrobacter succinogenes with Potential Roles in Adhesion to Cellulose and in Cellulose Digestion. J. Bacteriol. 189: 6806-6815 [Abstract] [Full Text]  
• Fieser, B. G., Vanzant, E. S. (2004). Interactions between supplement energy source and tall fescue hay maturity on forage utilization by beef steers. J ANIM SCI 82: 307-318 [Abstract] [Full Text]  
• Guedon, E., Desvaux, M., Petitdemange, H. (2002). Improvement of Cellulolytic Properties of Clostridium cellulolyticum by Metabolic Engineering. Appl. Environ. Microbiol. 68: 53-58 [Abstract] [Full Text]  
• Griffiths, E., Gupta, R. S. (2001). The use of signature sequences in different proteins to determine the relative branching order of bacterial divisions: evidence that Fibrobacter diverged at a similar time to Chlamydia and the Cytophaga-Flavobacterium-Bacteroides division. Microbiology 147: 2611-2622 [Abstract] [Full Text]  
• Desvaux, M., Guedon, E., Petitdemange, H. (2001). Carbon Flux Distribution and Kinetics of Cellulose Fermentation in Steady-State Continuous Cultures of Clostridium cellulolyticum on a Chemically Defined Medium. J. Bacteriol. 183: 119-130 [Abstract] [Full Text]  
• Desvaux, M., Guedon, E., Petitdemange, H. (2000). Cellulose Catabolism by Clostridium cellulolyticum Growing in Batch Culture on Defined Medium. Appl. Environ. Microbiol. 66: 2461-2470 [Abstract] [Full Text]  
• Guedon, E., Payot, S., Desvaux, M., Petitdemange, H. (1999). Carbon and Electron Flow in Clostridium cellulolyticum Grown in Chemostat Culture on Synthetic Medium. J. Bacteriol. 181: 3262-3269 [Abstract] [Full Text]