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Appl Environ Microbiol. 1990 March; 56(3): 808-811

Monoclonal antibody to type F Clostridium botulinum toxin.

J L Ferreira, M K Hamdy, S G McCay and F A Zapatka

Food and Drug Administration, Atlanta, Georgia 30309.

ABSTRACT

Hybridomas synthesizing monoclonal antibodies (MAbs) against type F Clostridium botulinum toxin were developed. MAb from one stable hybridoma, hybridoma 223, consisted of kappa light chains and an immunoglobulin G subclass 2a heavy chain. This MAb was used in a double-sandwich enzyme-linked immunosorbent assay to detect type F toxin in foods, culture fluids, and purified toxin preparations. The sensitivity of the double-sandwich enzyme-linked immunosorbent assay was approximately 10 mouse lethal doses of toxin per ml of toxic fluid.

Appl Environ Microbiol. 1990 March; 56(3): 808-811






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