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Appl Environ Microbiol. 1990 March; 56(3): 730-738
Copyright © 1990, American Society for Microbiology. All Rights Reserved.

HCO3– Fixation by Naturally Occurring Tufts and Pure Cultures of Thiothrix nivea

Michael F. McGlannan* and John C. Makemson

Department of Biological Sciences, Florida International University, Miami, Florida 33199

ABSTRACT

Naturally occurring tufts of the mixotroph Thiothrix nivea blanketed the East Everglades (Dade County, Fla.) Chekika artesian well and runoff areas. The rate of HCO3– fixation by these Thiothrix tufts was determined to be 14.0 ± 5.4 nmol of HCO3– per min per mg of dry weight, which reflected a growth rate of 5.0%/h. The addition of 10 mM glucose, ribose, acetate, or pyruvate or 0.05% Casamino Acids (Difco Laboratories, Detroit, Mich.) did not appear to alter the HCO3– fixation rate. Whereas 1 mM acetate or 10 mM lactate, ethanol, glycerol, {alpha}-ketoglutarate, succinate, fumarate, or citrate slightly stimulated HCO3– fixation, 5 to 10 mM malate inhibited HCO3– fixation by 90%. Pure Thiothrix cultures isolated from Chekika fixed HCO3– at rates as high as 29.9 ± 2.8 nmol of HCO3– per min per mg of dry weight in the presence of growth medium. Malate did not have a suppressive effect but rather slightly stimulated in vivo HCO3– fixation.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1990 March; 56(3): 730-738
Copyright © 1990, American Society for Microbiology. All Rights Reserved.



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