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Appl Environ Microbiol. 1990 February; 56(2): 569-571

Biotransformation of substituted benzoates to the corresponding cis-diols by an engineered strain of Pseudomonas oleovorans producing the TOL plasmid-specified enzyme toluate-1,2-dioxygenase.

Department of Medical Biochemistry, Faculty of Medicine, University of Geneva, Switzerland.

ABSTRACT

The conversion of substituted benzoates into 1,2-cis-dihydroxycyclohexa-3,5-diene carboxylic acids (cis-diols) was effected by using Escherichia coli and Pseudomonas recombinants carrying the xylXYZ genes originating from the Pseudomonas putida mt-2 TOL plasmid, thus producing toluate-1,2-dioxygenase. Pseudomonas oleovorans GPo12 recombinants readily produced meta- and para-substituted cis-diols, but were limited in their oxidation of ortho-substituted substrates.

This article has been cited by other articles:

• Ge, Y., Vaillancourt, F. H., Agar, N. Y. R., Eltis, L. D. (2002). Reactivity of Toluate Dioxygenase with Substituted Benzoates and Dioxygen. J. Bacteriol. 184: 4096-4103 [Abstract] [Full Text]