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Appl Environ Microbiol. 1990 February; 56(2): 466-471
Copyright © 1990, American Society for Microbiology. All Rights Reserved.

Diel Nitrogen Fixation by Cyanobacterial Surface Blooms in Sanctuary Lake, Pennsylvania

¹ Environmental Resources Center, State University of New York, College at Fredonia, Fredonia, New York 14063; Ecological Research Division, Office of Energy Research, U.S. Department of Energy, Washington, DC 20545²; and Department of Biology, Allegheny College, Meadville, Pennsylvania 16335³

ABSTRACT

Diel nitrogen fixation studies were conducted with assemblages of cyanobacteria sampled from surface blooms on Sanctuary Lake, Pa. The studies were conducted between July and September of 1982 to 1985 by using the acetylene reduction technique. Assemblages with the lowest cell concentrations (0.9 x 10⁹ to 1.0 x 10⁹ cells per liter) exhibited nitrogen fixation activity throughout the day, with maximum fixation rates occurring in mid to late afternoon; fixation proceeded throughout the night at rates equivalent to 23 to 28% of the afternoon maximum. In studies conducted with the highest cell concentrations (3.7 x 10⁹ to 6.7 x 10⁹ cells per liter), fixation rates reached maximum values in mid to late morning. The rates declined rapidly throughout the midday period and subsequently ceased from late afternoon until sunrise on the following day. The afternoon decline and cessation of fixation exhibited by high cell concentrations correlated with photosynthetically induced low total CO₂ and supersaturating O₂ concentrations. The midday decline could be prevented and partially reversed by experimentally lowering O₂ and increasing total CO₂ concentrations. Under experimental conditions which simultaneously prevented supersaturating O₂ concentrations and maintained high total CO₂ availability, nitrogen fixation continued throughout the solar day, with maximum rates occurring at midday. These observations indicate that temporal changes in photosynthetic activity may affect diel fluctuations in nitrogen fixation.

^* Corresponding author.