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Appl Environ Microbiol. 1990 December; 56(12): 3643-3648
Copyright © 1990, American Society for Microbiology. All Rights Reserved.

Degradation of Adsorbed Protein by Attached Bacteria in Relationship to Surface Hydrophobicity

Matts-Ola Samuelsson and David L. Kirchman^*

College of Marine Studies, University of Delaware, Lewes, Delaware 19958

ABSTRACT

The relationships among surface energy, adsorbed organic matter, and attached bacterial growth were examined by measuring the degradation of adsorbed ribulose-1,5-bisphosphate carboxylase (a common algal protein) by attached bacteria (Pseudomonas strain S9). We found that surface energy (work of adhesion of water) determined the amount and availability of adsorbed protein and, consequently, the growth of attached bacteria. Percent degradation of adsorbed ribulose-1,5-bisphosphate carboxylase decreased with increasing hydrophobicity of the surface (decreasing work of adhesion). As a result, growth rates of attached bacteria were initially higher on hydrophilic glass than on hydrophobic polyethylene. However, during long (6-h) incubations, growth rates increased with surface hydrophobicity because of increasing amounts of adsorbed protein. Together with previous studies, these results suggest that the number of attached bacteria over time will be a complex function of surface energy. Whereas both protein adsorption and bacterial attachment decrease with increasing surface energy, availability of adsorbed protein and consequently initial bacterial growth rates increase with surface energy.

^* Corresponding author.

Present address: Institutet för Vatten-och Luftvårdsforshning, Stockholm, Sweden.