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Appl Environ Microbiol. 1990 November; 56(11): 3285-3291
Copyright © 1990, American Society for Microbiology. All Rights Reserved.

Isolation and Identification of the Coal-Solubilizing Agent Produced by Trametes versicolor

Martin S. Cohen*, Kathleen A. Feldman, Cynthia S. Brown and Edward T. Gray Jr.

1 Department of Biology and Department of Chemistry, 2 University of Hartford, West Hartford, Connecticut 06117

ABSTRACT

Low-ranked coals were dissolved by using cell extracts derived from liquid cultures of Trametes versicolor. The coal-solubilizing agent (CSA) was separated from the broth components by a multistep isolation procedure including reverse-phase high-pressure liquid chromatography, size exclusion chromatography, ethanol fractionation, and recrystallization. Staircase voltammetry was used to show that two CSA moieties can coordinate to aqueous copper(II) ion. A molecular weight determination (using amperometry) gave an apparent molecular weight of 1.34 x 102 g/mol ± 8%. Nuclear magnetic resonance indicated that all protons on CSA are exchangeable in D2O and that there is only one type of carbon in CSA. The infrared spectrum of recrystallized CSA is identical to that of ammonium oxalate, and X-ray studies confirmed the crystal structure and composition of CSA to be that of ammonium oxalate monohydrate. The equivalent weight of the coal in solution, when the coal was dissolved by ammonium oxalate, is 7,940 g of coal per mol of iron present in the coal.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1990 November; 56(11): 3285-3291
Copyright © 1990, American Society for Microbiology. All Rights Reserved.



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