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Appl Environ Microbiol. 1990 November; 56(11): 3235-3239
Copyright © 1990, American Society for Microbiology. All Rights Reserved.

Effects of Partial O₂ Pressure, Partial CO₂ Pressure, and Agitation on Growth Kinetics of Azospirillum lipoferum under Fermentor Conditions

Etienne Paul, Daniel Mulard, Philippe Blanc, Jacques Fages, Gerard Goma and Alain Pareilleux^*

Département de Génie Biochimique et Alimentaire, URA-Centre National de la Recherche Scientifique, Institut National des Sciences Appliquées, Avenue de Rangueil, 31077 Toulouse Cédex, France

ABSTRACT

Azospirillum lipoferum crt1 was grown in batch cultures under standard conditions at 85% saturation of dissolved oxygen (DO) and 30-g/liter glucose concentrations. Kinetic studies revealed nutritional limitations of growth and the presence of an initial lag phase prior to consumption of glucose. The influences of various gaseous environments and shear stress on growth, i.e., various conditions of agitation-aeration, were characterized. Faster growth in the first stages of the culture and shorter duration of the lag phase were observed at DO concentrations of <30% saturation. The possible influences of dissolved CO₂ concentration or shear stress or both were discounted, and we confirmed the detrimental effect of high DO levels (up to 80% saturation) and the favorable influence of low DO concentrations (lower than 30% saturation) on growth. It was concluded that the gaseous environment, i.e., the DO concentration, needs to be considered as an operating parameter and be accurately controlled to ensure optimal growth of Azospirillum cells.

^* Corresponding author.

Present address: Pioneer France Maïs, Recherche Microbiologie Semences, F-31840 Aussonne, France.