This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Jepsen, P K Articles by Emborg, C Search for Related Content PubMed PubMed Citation Articles by Jepsen, P K Articles by Emborg, C Agricola Articles by Jepsen, P K Articles by Emborg, C

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1987 October; 53(10): 2593-2596

Two-level factorial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease.

Department of Biotechnology, Technical University of Denmark, Lyngby.

ABSTRACT

Two high-nuclease-yielding mutants of Serratia marcescens, derived by chemical mutagenesis (W280, W355), and two strains with the pBR322 plasmid 403-SD2, carrying a nuclease gene and a chloramphenicol resistance gene [Escherichia coli CSH50(403-SD2) and S. marcescens CH30(403-SD2)] were investigated for nuclease production in a factorial shake flask experiment, with temperature (30 and 37 degrees C), pH (with or without CaCO3 tablets), and aeration (with or without baffles) as variable conditions. Yields varied 10-fold depending on the conditions investigated.