This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kouker, G Articles by Jaeger, K E Search for Related Content PubMed PubMed Citation Articles by Kouker, G Articles by Jaeger, K E Agricola Articles by Kouker, G Articles by Jaeger, K E

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1987 January; 53(1): 211-213

Specific and sensitive plate assay for bacterial lipases.

ABSTRACT

A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby allowing quantitation of lipase activities ranging from 1 to 30 nkat.

This article has been cited by other articles:

• An, D.-S., Im, W.-T., Yoon, M.-H. (2008). Microlunatus panaciterrae sp. nov., a {beta}-glucosidase-producing bacterium isolated from soil in a ginseng field. Int. J. Syst. Evol. Microbiol. 58: 2734-2738 [Abstract] [Full Text]  
• Cai, Z., Xu, W., Xue, R., Lin, Z. (2008). Facile, reagentless and in situ release of Escherichia coli intracellular enzymes by heat-inducible autolytic vector for high-throughput screening. Protein Eng Des Sel 21: 681-687 [Abstract] [Full Text]  
• Lee, M., Ten, L. N., Lee, H.-W., Oh, H. W., Im, W.-T., Lee, S.-T. (2008). Sphingopyxis ginsengisoli sp. nov., isolated from soil of a ginseng field in South Korea. Int. J. Syst. Evol. Microbiol. 58: 2342-2347 [Abstract] [Full Text]  
• Im, W.-T., Jung, H.-M., Ten, L. N., Kim, M. K., Bora, N., Goodfellow, M., Lim, S., Jung, J., Lee, S.-T. (2008). Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge. Int. J. Syst. Evol. Microbiol. 58: 2348-2353 [Abstract] [Full Text]  
• Liu, Q.-M., Ten, L. N., Im, W.-T., Lee, S.-T. (2008). Castellaniella caeni sp. nov., a denitrifying bacterium isolated from sludge of a leachate treatment plant. Int. J. Syst. Evol. Microbiol. 58: 2141-2146 [Abstract] [Full Text]  
• Yoon, M.-H., Ten, L. N., Im, W.-T., Lee, S.-T. (2008). Cellulomonas chitinilytica sp. nov., a chitinolytic bacterium isolated from cattle-farm compost. Int. J. Syst. Evol. Microbiol. 58: 1878-1884 [Abstract] [Full Text]  
• Liu, Q.-M., Ten, L. N., Yu, H.-S., Jin, F.-X., Im, W.-T., Lee, S.-T. (2008). Emticicia ginsengisoli sp. nov., a species of the family 'Flexibacteraceae' isolated from soil of a ginseng field. Int. J. Syst. Evol. Microbiol. 58: 1100-1105 [Abstract] [Full Text]  
• Gil, F., Catalao, M. J., Moniz-Pereira, J., Leandro, P., McNeil, M., Pimentel, M. (2008). The lytic cassette of mycobacteriophage Ms6 encodes an enzyme with lipolytic activity. Microbiology 154: 1364-1371 [Abstract] [Full Text]  
• Park, M.-J., Kim, M. K., Kim, H.-B., Im, W.-T., Yi, T.-H., Kim, S.-Y., Soung, N.-K., Yang, D.-C. (2008). Microbacterium ginsengisoli sp. nov., a {beta}-glucosidase-producing bacterium isolated from soil of a ginseng field. Int. J. Syst. Evol. Microbiol. 58: 429-433 [Abstract] [Full Text]  
• Ten, L. N., Baek, S.-H., Im, W.-T., Larina, L. L., Lee, J.-S., Oh, H.-M., Lee, S.-T. (2007). Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field. Int. J. Syst. Evol. Microbiol. 57: 2532-2537 [Abstract] [Full Text]  
• Lee, H.-W., Yu, H.-S., Liu, Q.-m., Jung, H.-M., An, D.-S., Im, W.-T., Jin, F.-X., Lee, S.-T. (2007). Kaistia granuli sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int. J. Syst. Evol. Microbiol. 57: 2280-2283 [Abstract] [Full Text]  
• Yoon, M.-H., Ten, L. N., Im, W.-T., Lee, S.-T. (2007). Methylibium fulvum sp. nov., a member of the Betaproteobacteria isolated from ginseng field soil, and emended description of the genus Methylibium. Int. J. Syst. Evol. Microbiol. 57: 2062-2066 [Abstract] [Full Text]  
• Yoon, M.-H., Ten, L. N., Im, W.-T. (2007). Paenibacillus ginsengarvi sp. nov., isolated from soil from ginseng cultivation. Int. J. Syst. Evol. Microbiol. 57: 1810-1814 [Abstract] [Full Text]  
• Yoon, M.-H., Im, W.-T. (2007). Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil. Int. J. Syst. Evol. Microbiol. 57: 1834-1839 [Abstract] [Full Text]  
• Jin, L., Kim, K. K., Im, W.-T., Yang, H.-C., Lee, S.-T. (2007). Aspromonas composti gen. nov., sp. nov., a novel member of the family Xanthomonadaceae. Int. J. Syst. Evol. Microbiol. 57: 1876-1880 [Abstract] [Full Text]  
• Lee, H.-G., An, D.-S., Im, W.-T., Liu, Q.-M., Na, J.-R., Cho, D. H., Jin, C. W., Lee, S.-T., Yang, D.-C. (2007). Chitinophaga ginsengisegetis sp. nov. and Chitinophaga ginsengisoli sp. nov., isolated from soil of a ginseng field in South Korea. Int. J. Syst. Evol. Microbiol. 57: 1396-1401 [Abstract] [Full Text]  
• Kang, M.-S., Im, W.-T., Jung, H.-M., Kim, M. K., Goodfellow, M., Kim, K. K., Yang, H.-C., An, D.-S., Lee, S.-T. (2007). Cellulomonas composti sp. nov., a cellulolytic bacterium isolated from cattle farm compost. Int. J. Syst. Evol. Microbiol. 57: 1256-1260 [Abstract] [Full Text]  
• Ruiz, C., Falcocchio, S., Pastor, F. I. J., Saso, L., Diaz, P. (2007). Helicobacter pylori EstV: Identification, Cloning, and Characterization of the First Lipase Isolated from an Epsilon-Proteobacterium. Appl. Environ. Microbiol. 73: 2423-2431 [Abstract] [Full Text]  
• Cui, Y.-S., Im, W.-T., Yin, C.-R., Lee, J.-S., Lee, K. C., Lee, S.-T. (2007). Aeromicrobium panaciterrae sp. nov., isolated from soil of a ginseng field in South Korea. Int. J. Syst. Evol. Microbiol. 57: 687-691 [Abstract] [Full Text]  
• Cui, Y.-S., Im, W.-T., Yin, C.-R., Yang, D.-C., Lee, S.-T. (2007). Microlunatus ginsengisoli sp. nov., isolated from soil of a ginseng field. Int. J. Syst. Evol. Microbiol. 57: 713-716 [Abstract] [Full Text]  
• de Eugenio, L. I., Garcia, P., Luengo, J. M., Sanz, J. M., Roman, J. S., Garcia, J. L., Prieto, M. A. (2007). Biochemical Evidence That phaZ Gene Encodes a Specific Intracellular Medium Chain Length Polyhydroxyalkanoate Depolymerase in Pseudomonas putida KT2442: CHARACTERIZATION OF A PARADIGMATIC ENZYME. J. Biol. Chem. 282: 4951-4962 [Abstract] [Full Text]  
• Yoon, M.-H., Ten, L. N., Im, W.-T., Lee, S.-T. (2007). Pedobacter panaciterrae sp. nov., isolated from soil in South Korea. Int. J. Syst. Evol. Microbiol. 57: 381-386 [Abstract] [Full Text]  
• Park, M.-J., Kim, H.-B., An, D.-S., Yang, H.-C., Oh, S.-T., Chung, H.-J., Yang, D.-C. (2007). Paenibacillus soli sp. nov., a xylanolytic bacterium isolated from soil. Int. J. Syst. Evol. Microbiol. 57: 146-150 [Abstract] [Full Text]  
• Ten, L. N., Baek, S.-H., Im, W.-T., Liu, Q.-M., Aslam, Z., Lee, S.-T. (2006). Bacillus panaciterrae sp. nov., isolated from soil of a ginseng field. Int. J. Syst. Evol. Microbiol. 56: 2861-2866 [Abstract] [Full Text]  
• Kim, H.-B., Park, M.-J., Yang, H.-C., An, D.-S., Jin, H.-Z., Yang, D.-C. (2006). Burkholderia ginsengisoli sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.. Int. J. Syst. Evol. Microbiol. 56: 2529-2533 [Abstract] [Full Text]  
• Ten, L. N., Liu, Q.-M., Im, W.-T., Lee, M., Yang, D.-C., Lee, S.-T. (2006). Pedobacter ginsengisoli sp. nov., a DNase-producing bacterium isolated from soil of a ginseng field in South Korea.. Int. J. Syst. Evol. Microbiol. 56: 2565-2570 [Abstract] [Full Text]  
• Baek, S.-H., Im, W.-T., Oh, H. W., Lee, J.-S., Oh, H.-M., Lee, S.-T. (2006). Brevibacillus ginsengisoli sp. nov., a denitrifying bacterium isolated from soil of a ginseng field.. Int. J. Syst. Evol. Microbiol. 56: 2665-2669 [Abstract] [Full Text]  
• Ten, L. N., Baek, S.-H., Im, W.-T., Lee, M., Oh, H. W., Lee, S.-T. (2006). Paenibacillus panacisoli sp. nov., a xylanolytic bacterium isolated from soil in a ginseng field in South Korea.. Int. J. Syst. Evol. Microbiol. 56: 2677-2681 [Abstract] [Full Text]  
• Kim, K.-H., Ten, L. N., Liu, Q.-M., Im, W.-T., Lee, S.-T. (2006). Sphingobacterium daejeonense sp. nov., isolated from a compost sample.. Int. J. Syst. Evol. Microbiol. 56: 2031-2036 [Abstract] [Full Text]  
• Liu, Q.-M., Im, W.-T., Lee, M., Yang, D.-C., Lee, S.-T. (2006). Dyadobacter ginsengisoli sp. nov., isolated from soil of a ginseng field.. Int. J. Syst. Evol. Microbiol. 56: 1939-1944 [Abstract] [Full Text]  
• Quan, Z.-X., Im, W.-T., Lee, S.-T. (2006). Azonexus caeni sp. nov., a denitrifying bacterium isolated from sludge of a wastewater treatment plant.. Int. J. Syst. Evol. Microbiol. 56: 1043-1046 [Abstract] [Full Text]  
• Cirne, D. G., Delgado, O. D., Marichamy, S., Mattiasson, B. (2006). Clostridium lundense sp. nov., a novel anaerobic lipolytic bacterium isolated from bovine rumen.. Int. J. Syst. Evol. Microbiol. 56: 625-628 [Abstract] [Full Text]  
• An, D.-S., Im, W.-T., Yang, H.-C., Lee, S.-T. (2006). Shinella granuli gen. nov., sp. nov., and proposal of the reclassification of Zoogloea ramigera ATCC 19623 as Shinella zoogloeoides sp. nov.. Int. J. Syst. Evol. Microbiol. 56: 443-448 [Abstract] [Full Text]  
• Yang, H.-C., Im, W.-T., Kim, K. K., An, D.-S., Lee, S.-T. (2006). Burkholderia terrae sp. nov., isolated from a forest soil. Int. J. Syst. Evol. Microbiol. 56: 453-457 [Abstract] [Full Text]  
• Yang, H.-C., Im, W.-T., Kang, M. S., Shin, D.-Y., Lee, S.-T. (2006). Stenotrophomonas koreensis sp. nov., isolated from compost in South Korea. Int. J. Syst. Evol. Microbiol. 56: 81-84 [Abstract] [Full Text]  
• Feng, J., Liu, G., Selvaraj, G., Hughes, G. R., Wei, Y. (2005). A secreted lipase encoded by LIP1 is necessary for efficient use of saturated triglyceride lipids in Fusarium graminearum. Microbiology 151: 3911-3921 [Abstract] [Full Text]  
• Yang, H.-C., Im, W.-T., An, D.-S., Park, W.-s., Kim, I. S., Lee, S.-T. (2005). Silvimonas terrae gen. nov., sp. nov., a novel chitin-degrading facultative anaerobe belonging to the 'Betaproteobacteria'. Int. J. Syst. Evol. Microbiol. 55: 2329-2332 [Abstract] [Full Text]  
• An, D.-S., Im, W.-T., Yang, H.-C., Yang, D.-C., Lee, S.-T. (2005). Dyella koreensis sp. nov., a {beta}-glucosidase-producing bacterium. Int. J. Syst. Evol. Microbiol. 55: 1625-1628 [Abstract] [Full Text]  
• An, D.-S., Im, W.-T., Yang, H.-C., Kang, M. S., Kim, K. K., Jin, L., Kim, M. K., Lee, S.-T. (2005). Cellulomonas terrae sp. nov., a cellulolytic and xylanolytic bacterium isolated from soil. Int. J. Syst. Evol. Microbiol. 55: 1705-1709 [Abstract] [Full Text]  
• Lee, S. H., Choi, J.-I., Park, S. J., Lee, S. Y., Park, B. C. (2004). Display of Bacterial Lipase on the Escherichia coli Cell Surface by Using FadL as an Anchoring Motif and Use of the Enzyme in Enantioselective Biocatalysis. Appl. Environ. Microbiol. 70: 5074-5080 [Abstract] [Full Text]  
• Schlesner, H., Rensmann, C., Tindall, B. J., Gade, D., Rabus, R., Pfeiffer, S., Hirsch, P. (2004). Taxonomic heterogeneity within the Planctomycetales as derived by DNA-DNA hybridization, description of Rhodopirellula baltica gen. nov., sp. nov., transfer of Pirellula marina to the genus Blastopirellula gen. nov. as Blastopirellula marina comb. nov. and emended description of the genus Pirellula. Int. J. Syst. Evol. Microbiol. 54: 1567-1580 [Abstract] [Full Text]  
• Miteva, V. I., Sheridan, P. P., Brenchley, J. E. (2004). Phylogenetic and Physiological Diversity of Microorganisms Isolated from a Deep Greenland Glacier Ice Core. Appl. Environ. Microbiol. 70: 202-213 [Abstract] [Full Text]  
• Murphy, J L, Badaloo, A V, Chambers, B, Forrester, T E, Wootton, S A, Jackson, A A (2002). Maldigestion and malabsorption of dietary lipid during severe childhood malnutrition. Arch. Dis. Child. 87: 522-525 [Abstract] [Full Text]  
• Bell, P. J. L., Sunna, A., Gibbs, M. D., Curach, N. C., Nevalainen, H., Bergquist, P. L. (2002). Prospecting for novel lipase genes using PCR. Microbiology 148: 2283-2291 [Abstract] [Full Text]  
• Kim, E. K., Jang, W. H., Ko, J. H., Kang, J. S., Noh, M. J., Yoo, O. J. (2001). Lipase and Its Modulator from Pseudomonas sp. Strain KFCC 10818: Proline-to-Glutamine Substitution at Position 112 Induces Formation of Enzymatically Active Lipase in the Absence of the Modulator. J. Bacteriol. 183: 5937-5941 [Abstract] [Full Text]  
• Henne, A., Schmitz, R. A., Bömeke, M., Gottschalk, G., Daniel, R. (2000). Screening of Environmental DNA Libraries for the Presence of Genes Conferring Lipolytic Activity on Escherichia coli. Appl. Environ. Microbiol. 66: 3113-3116 [Abstract] [Full Text]  
• Choo, D.-W., Kurihara, T., Suzuki, T., Soda, K., Esaki, N. (1998). A Cold-Adapted Lipase of an Alaskan Psychrotroph, Pseudomonas sp. Strain B11-1: Gene Cloning and Enzyme Purification and Characterization. Appl. Environ. Microbiol. 64: 486-491 [Abstract] [Full Text]  
• Handrick, R., Reinhardt, S., Focarete, M. L., Scandola, M., Adamus, G., Kowalczuk, M., Jendrossek, D. (2001). A New Type of Thermoalkalophilic Hydrolase of Paucimonas lemoignei with High Specificity for Amorphous Polyesters of Short Chain-length Hydroxyalkanoic Acids. J. Biol. Chem. 276: 36215-36224 [Abstract] [Full Text]