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Appl Environ Microbiol. 1985 December; 50(6): 1357-1360
Copyright © 1985, American Society for Microbiology. All Rights Reserved.

Diffusion through a Double-Sided Plate: Development of a Method to Study Alga-Bacterium Interactions

Frederick S. Colwell{dagger},* and Harold K. Speidel

Department of Biology, Northern Arizona University, Flagstaff, Arizona 86011

ABSTRACT

Bacteria and algae isolated from a wastewater oxidation pond were inoculated onto opposing surfaces of double-layer agar plates (Lutri plates) to determine the usefulness of such plates for studying microbial interactions. The altered growth characteristics of various algae depending on the species of bacteria on the adjacent medium surface indicated that there was diffusion of extracellular products through the agar, suggesting that this simple assay can be used for screening potential interactions of actively growing organisms.

FOOTNOTES

* Corresponding author.

{dagger} Present address: Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061.

Appl Environ Microbiol. 1985 December; 50(6): 1357-1360
Copyright © 1985, American Society for Microbiology. All Rights Reserved.



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Copyright © 1985 by the American Society for Microbiology. All rights reserved.