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Appl Environ Microbiol. 1985 May; 49(5): 1251-1253

An instrument for the immediate quantification of bacteria in potable waters.

ABSTRACT

A new semiautomated instrument is described which quantifies the number of bacteria in potable waters within 3 min, providing a permanent colorimetric record of the results. The bacteria detection device can measure as few as 100 CFU/ml in potable waters. In brief, a 100- to 1,000-ml sample of tap water is drawn through a large surface, customized filter housed in the device, and bacteria, rust, and humic acid in the water are concentrated thereon. A reducing agent is used to remove the rust and humic acid from the filter. The filter is inverted and backflushed to elute the bacteria which are collected and reconcentrated onto a 7-mm-diameter filter surface. The reconcentrated bacteria are stained, and the filter fibers are preferentially decolorized without removing the dye from the bacteria. The color intensity of the filter surface is compared with a color guide to determine the amount of bacteria in the test water.