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Appl Environ Microbiol. 1971 September; 22(3): 387-392
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

L-Asparaginase from Proteus vulgaris1

Tetsuya Tosa, Ryujiro Sano, Kozo Yamamoto, Masatoshi Nakamura, Katsuko Ando and Ichiro Chibata

Department of Applied Biochemistry, Tanabe Seiyaku Co. Ltd., Kashima-cho, Higashiyodogawa-ku, Osaka, Japan
Chemical Research Laboratory, Tanabe Seiyaku Co. Ltd., Kashima-cho, Higashiyodogawa-ku, Osaka, Japan

ABSTRACT

To produce an immunologically and enzymologically new type of L-asparaginase, 108 strains of bacteria were screened for enzyme production. As a result, 13 bacteria belonging to the genera Alcaligenes, Bacterium, and Proteus were found to produce L-asparaginases in high levels. Among these L-asparaginases, partially purified L-asparaginases from B. cadaveris and P. vulgaris showed antitumor activity. A partially purified L-asparaginase preparation of P. vulgaris did not react with the antibody of Escherichia coliL-asparaginase on the Ouchterlony agar plate. Culture conditions for the production of L-asparaginase by P. vulgaris were investigated in detail. The enzyme was produced in high yields when cells were grown aerobically in a medium containing sodium fumarate and corn steep liquor. The addition of glucose or ammonium ion to the medium, however, resulted in depressed production of L-asparaginase. Under the optimum conditions, 3,700 international units of L-asparaginase was obtained from 1 liter of culture medium.

FOOTNOTES

1 A part of this investigation was presented at the Annual Meeting of the Agricultural Chemistry of Japan, Fukuoka, Japan, 4 April 1970.

Appl Environ Microbiol. 1971 September; 22(3): 387-392
Copyright © 1971 American Society for Microbiology. All Rights Reserved.





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Copyright © 1971 by the American Society for Microbiology. All rights reserved.