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Appl Environ Microbiol. 1971 September; 22(3): 366-371
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Biochemical Properties of Haploid and Diploid Strains of Penicillium chrysogenum

S. G. Pathak and R. P. Elander

Wyeth Laboratories, Inc., West Chester, Pennsylvania 19380
Antibiotic Division, West Chester, Pennsylvania 19380

ABSTRACT

An intensive parasexual genetics program in which industrial strains of Penicillium chrysogenum were used culminated in the isolation of a number of heterozygous diploid strains. The diploid clones were selected from heterokaryons formed from matings between mutant strains having complementary biochemical and conidial color markers. Several diploid cultures were compared with their haploid wild-type parents and other distantly related production strains on the basis of a variety of cultural and physiological criteria. The diploid strains characteristically produced conidia of larger volume and higher deoxyribonucleic acid content. Some were vigorous with respect to growth rate and onset and degree of conidiation. One diploid strain (WC-9) had a 46% greater oxygen uptake rate and oxidized glucose at a 57% greater rate than its haploid parent (M-2). It also produced 33% higher concentrations of ß-galactosidase, 66% more alkaline protease, and 53% more glucose oxidase than the M-2 haploid parent. The selection of rare stable diploid mold cultures through the use of parasexual genetics offers a unique approach to the direct selection of mutants with potential for increased enzyme formation.

Appl Environ Microbiol. 1971 September; 22(3): 366-371
Copyright © 1971 American Society for Microbiology. All Rights Reserved.





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Copyright © 1971 by the American Society for Microbiology. All rights reserved.