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Appl Environ Microbiol. 1971 September; 22(3): 267-269
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Evaluation of Accuracy of Multitest Micromethod System for Identification of Enterobacteriaceae

Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55901

ABSTRACT

The Analytab system of 20 biochemical tests for identification of Enterobacteriaceae was evaluated in parallel with conventional tests on 128 Enterobacteriaceae, 5 Aeromonas, and 1 Yersinia enterocolitica. The results of tests for H₂S and indole production, citrate utilization, lysine and ornithine decarboxylase, arginine dihydrolase, nitrate reduction, ß-galactosidase, and fermentation of arabinose, rhamnose, mannitol, and glucose showed almost complete agreement between the two systems. Eighty-eight per cent of Enterobacteriaceae were correctly speciated with the Analytab system; on repeat testing with heavier inocula of organisms failing to ferment glucose initially, the proportion of Enterobacteriaceae correctly speciated became 93%.