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Appl Environ Microbiol. 1971 August; 22(2): 214-219
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Analysis of Staphylococcal Enterotoxin B by the Polyacrylamide Electrophoresis Technique

Medical Sciences Laboratories, Fort Detrick, Frederick, Maryland 21701

ABSTRACT

The electrophoretic mobility of enterotoxin B was investigated through the use of the disc electrophoresis technique. Ideal patterns were developed with a 7.5% acrylamide gel system (pH 4.3). The toxin can be separated and identified from other complex proteins such as serum or suspect samples of foods by this technique. The technique can be used as an assay method for the toxin as well as to elucidate physical changes in the toxin due to temperature. The method should not be considered exclusive for enterotoxin B.