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Appl Environ Microbiol. 1971 July; 22(1): 79-83
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Preparation of Labeled Aflatoxins with High Specific Activities

Department of Environmental Toxicology, University of California, Davis, California 95616
Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

ABSTRACT

Resting cells of Aspergillus parasiticus ATCC 15517 were used to prepare highly labeled aflatoxins from labeled acetate. High synthetic activity in growing cells was evidenced only during 40 to 70 hr of incubation. Glucose was required for high incorporation efficiency, whereas the concentration of the labeled acetate determined the specific activity of the product. When labeled acetate was continuously added to maintain a concentration near but not exceeding 10 mM, in a culture containing 30 g of glucose per liter, 2% of its labels could be recovered in the purified aflatoxins which have a specific activity more than three times that of the labeled acetate.

¹ Present address: The Hebrew University-Hadassah Medical School, P.O. Box 1172, Jerusalem, Israel.