This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Dunn, C. Articles by Martin, W. J. Search for Related Content PubMed Articles by Dunn, C. Articles by Martin, W. J. Agricola Articles by Dunn, C. Articles by Martin, W. J.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1971 July; 22(1): 17-22
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Comparison of Media for Isolation of Salmonellae and Shigellae from Fecal Specimens ¹

Enteric Bacteriology Unit, Center for Disease Control, Atlanta, Georgia 30333

ABSTRACT

Five transport media, eight plating media, and three enrichment broth media for the isolation of salmonellae and shigellae were evaluated. Eight laboratories in widely separated regions of the United States participated in this evaluation by submitting 490 fecal specimens in the transport media provided. The results suggest that the newer transport media may not offer any advantage over the use of buffered glycerol-saline in the isolation of these enteric pathogens. Shigellae were best isolated by direct inoculation, whereas salmonellae were isolated in greater numbers after tetrathionate (without Brilliant Green) enrichment with subsequent culturing on the plating medium. The use of a variety of plating media is recommended for the recovery of a larger number of these enteric pathogens.

² Present address: Department of Internal Medicine, University of Cincinnati, College of Medicine, Cincinnati, Ohio 45229.

³ Present address: Department of Microbiology and Immunology, Mayo Clinic and Foundation, Rochester, Minn. 55901.

¹ Presented in part at the 70th Annual Meeting of the American Society for Microbiology, Boston, 26 April to 1 May, 1970.