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Appl Environ Microbiol. 1971 July; 22(1): 145-146
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Growth of Human Lymphoid Cells (Raji Strain) in a Five-Liter Fermentor

Frederick Klein, Bill G. Mahlandt and Ralph E. Lincoln

Process Development Division, Department of the Army, Fort Detrick, Frederick, Maryland 27101

ABSTRACT

Procedures used to produce an established line of mammalian cells (L cell) in a New Brunswick fermentor were adapted to propagate human lymphoid cells in suspended culture. Control of pH within defined limits was more effective for regulation of cell metabolism than control of oxidation-reduction potential. An unusually high rate of agitation was required.

Appl Environ Microbiol. 1971 July; 22(1): 145-146
Copyright © 1971 American Society for Microbiology. All Rights Reserved.





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Copyright © 1971 by the American Society for Microbiology. All rights reserved.