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Appl Environ Microbiol. 1971 July; 22(1): 135-136
Copyright © 1971 American Society for Microbiology. All Rights Reserved.
Medical Sciences Laboratories, Fort Detrick, Frederick, Maryland 21701
ABSTRACT
Accumulation of protective antigen was essentially independent of culture volume, provided the organisms were kept in suspension by stirring. Agitation by orbited shaking was unsatisfactory.
1 Present address: Electronucleonics Inc., Bethesda, Md.
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