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Appl Environ Microbiol. 1971 July; 22(1): 110-112
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Determination of Hydrogen Sulfide in Fermentation Broths Containing SO₂¹

Department of Food Science and Technology, New York State Agricultural Experiment Station, Cornell University, Geneva, New York 14456

ABSTRACT

A procedure for the determination of hydrogen sulfide in fermentation broths containing up to 100 µg of SO₂ per ml is described. The method involves the sparging of H₂S from the broth into a cadmium hydroxide absorption solution, the formation of methylene blue from the absorbed sulfide, and the measuring of this color spectrophotometrically. The use of cadmium hydroxide instead of zinc acetate, the common absorbent, substantially reduced the interference of SO₂ with the analysis.

¹ Approved by the Director of the New York State Agricultural Experiment Station for publication as Journal paper no. 1878.