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Appl Environ Microbiol. 1971 June; 21(6): 1075-1079
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Production of Anticapsin by Streptomyces griseoplanus¹

Fermentation Products Research, Eli Lilly and Company, Indianapolis, Indiana 46206

ABSTRACT

Anticapsin is a new fermentation product which inhibits formation of the hyaluronic acid capsule of Streptococcus pyogenes. Production of this metabolite in a complex medium by S. griseoplanus is enhanced by high levels of carbohydrate. A number of carbon sources support biosynthesis but sucrose is most effective, the optimum level being 150 g/liter. Neither glucose nor fructose, alone or in combination, serves as an equivalent substitute for sucrose. The addition of dibasic potassium phosphate to the medium further increases anticapsin production. Dissolved oxygen levels are important for synthesis and stability of the metabolite. Anticapsular activity diminishes rapidly in previously aerated broths which are held under static conditions. This decrease does not occur in pasteurized broths or unpasteurized filtrates.

¹ Presented in part at the 70th Annual Meeting of the American Society for Microbiology, Boston, Mass., 26 April to 1 May 1970.