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Appl Environ Microbiol. 1971 June; 21(6): 1040-1045
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Microorganisms in Unamended Soil as Observed by Various Forms of Microscopy and Staining ¹

Department of Microbiology, The Pennsylvania State University, University Park, Pennsylvania 16802

ABSTRACT

A light-diffraction microscope was modified to allow sequential viewing of the microorganisms in a soil smear by transmitted, reflected, and reflected-polarized incandescent light and by reflected ultraviolet light. Observations were also made by conventional incandescent and ultraviolet transmitted-light microscopy. All results for the various forms of bright-field microscopy with stained and unstained soils were in agreement, but they differed from the results obtained for two types of ultraviolet-fluorescence microscopy. The latter proved to be nonspecific for in situ soil microorganisms. Capsule-like areas were noted surrounding many of the resident microbial cells of soil when viewed by the various forms of bright-field microscopy. These areas could not be stained or removed by a variety of treatments, but they apparently often did take up stain after in situ soil growth had been initiated. It was concluded that these areas are not capsules but may represent a structural component of nonmultiplying microbial cells in soil.

¹ This research was authorized for publication as paper no. 3939 in the journal series of the Pennsylvania Agricultural Experiment Station on 11 March 1971.