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Appl Environ Microbiol. 1971 June; 21(6): 1002-1006
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Specific Assay of Aminoglycosidic- or Polymyxin-Type Antibiotics Present in Human Sera in Combination with Cephalosporins

The Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46206

ABSTRACT

Monitoring of serum concentrations of aminoglycosidic or polymyxin antibiotics when administered concurrently with cephalosporins or penicillins requires a special assay technique. Selective enzymatic degradation of the ß-lactam antibiotic from the serum specimen allows subsequent assay of the antibiotic being monitored. This report gives details of a simple procedure for laboratory production of a crude enzyme capable of degrading cephalosporins or penicillins. An assay procedure for quantitating aminoglycosidic or polymyxin antibiotics after enzymatic degradation of a coexisting ß-lactam antibiotic is described.