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Appl Environ Microbiol. 1971 May; 21(5): 893-898
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Disc-Agar Diffusion Microbiological Assay Procedure for Determining Serum and Urine Levels of Sulfacytine and Other Sulfonamides

Biological Research and Development Department, Medical and Scientific Affairs Division, Parke, Davis & Company, Detroit, Michigan 48232

ABSTRACT

A reasonably precise, reproducible, and sensitive microbiological procedure for directly assaying sulfacytine and other sulfonamides as antibacterially active drugs has been developed by appropriately modifying the standard disc-agar diffusion technique. Blood and urine levels as low as 3 µg/ml may be determined through the use of a strain of Escherichia coli and a chemically defined agar medium devoid of sulfonamide antagonists. Results indicate that this assay method should be a useful adjunct to the Bratton-Marshall colorimetric procedure, by permitting the direct measurement of antibacterially active drug in clinical specimens.