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Appl Environ Microbiol. 1971 May; 21(5): 867-869
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Enhanced Detection of Australia Antigen in Serum Hepatitis Patients by Discontinuous Counter-Immunoelectrophoresis

Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77025

ABSTRACT

The use of discontinuous counter-immunoelectrophoresis enhanced the reaction between Au/SH antigen and its antibody in agarose. The ionic strength of the Veronal buffer used in the agarose was 0.015 µ, whereas 0.075 µ Veronal (both pH 8.6) was used for anode and cathode buffers. Electroendosmosis is increased under such conditions. Au/SH antigen and antibody reacted to give sharp lines within 30 to 45 min as compared with conventional counter-immunoelectrophoresis which required 1 to 3 hr or longer.