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Appl Environ Microbiol. 1971 May; 21(5): 852-853
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Microflocculation Assay for Gonococcal Antibody

Venereal Disease Research Laboratory, Center for Disease Control, Atlanta, Georgia 30333

ABSTRACT

An antigen suspension consisting of cholesterol-lecithin particles sensitized with an extract of gonococci was used in a flocculation assay for the detection of human antibodies to Neisseria gonorrhoeae. With culturally and clinically diagnosed cases of uncomplicated gonorrhea, sera from 70 (49.6%) of 141 males and 207 (79.0%) of 262 females were reactive in the assay. Sera from four (7.3%) of 55 males and 11 (5.4%) of 203 females, all presumed normal, were reactive. The assay utilized equipment available in most clinical and public health laboratories.