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Appl Environ Microbiol. 1971 April; 21(4): 600-605
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Identification of Mycoplasmatales: Characterization Procedures ¹

Department of Avian Diseases, New York State Veterinary College, Cornell University, Ithaca, New York 14850

ABSTRACT

A large collection of avian Mycoplasma cultures was used in studies to improve and develop biological and biochemical characterization techniques. Differential patterns among 11 avian serotypes were shown by carbohydrate fermentation, tetrazolium- and methylene blue-reduction reactions, breakdown of arginine, and the formation of film on egg yolk-agar. Some cultures fermented as many as 14 carbohydrates. Polyhydric alcohols and pentoses were among the compounds fermented. An improved procedure for determining methylene blue reduction by Mycoplasma was developed. These simple, rapid procedures are reproducible and should be useful in grouping Mycoplasma isolates prior to definitive identification by serological or other means.

² Present address: Animal Disease Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Denver Federal Center, Bldg. 45-B, Denver, Colo. 80225.

Submitted by the senior author to Cornell University, Ithaca, N.Y., in partial fulfillment of the requirements for the Ph.D. Degree.