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Appl Environ Microbiol. 1971 April; 21(4): 588-593
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Free and Immobilized Glucose Isomerase from Streptomyces phaeochromogenes¹

Northern Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604

ABSTRACT

Properties were determined of the glucose isomerase from Streptomyces phaeochromogenes NRRL B-3559. The enzyme exhibited a temperature optimum of 80 C and a pH optimum of about 8. The effect of various buffers on activity of the enzyme and the optimum pH were studied. Michaelis constants for glucose and Mg²⁺ were 0.25 and 0.025 M, respectively. Co²⁺ enhanced enzyme activity. A functional polyacrylamide-entrapped glucose isomerase was prepared. The conditions for entrapment and use of the bound enzyme were examined.

¹ Presented, in part, at the American Society for Microbiology meeting, Miami Beach, Fla., May 1969.