This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Nagel, C. W. Articles by Wilson, T. M. Search for Related Content PubMed Articles by Nagel, C. W. Articles by Wilson, T. M. Agricola Articles by Nagel, C. W. Articles by Wilson, T. M.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1970 September; 20(3): 374-383
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Pectic Acid Lyases of Bacillus polymyxa¹

Food Sciences Program, Department of Horticulture, Washington State University, Pullman, Washington 99163

ABSTRACT

Four enzymes were separated from an extracellular preparation of Bacillus polymyxa by carboxymethylcellulose column chromatography. The pH optima were 8.3 to 8.5, 8.7 to 8.9, 9.2 to 9.4, and 9.5 to 9.6. All of the enzymes required calcium ion for maximum activity, whereas strontium ion was only partially effective in stimulating activity. Cobalt was the only other cation tested which was effective in two of the enzymes. The lyases seem to attack a calcium salt-bridged substrate. K_m and V_m data of the four enzymes on various oligomers are presented as well as paper chromatographic evidence of preferred sites of attack. All of the enzymes are endo-enzymes which, based upon their characteristics, were classed into two types.

¹ Scientific paper no. 3380, College of Agriculture, Washington State University, Pullman, project no. 1620. This investigation was supported by Public Health Service grant GM-08088 from the Division of Research Grants.