AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hamdy, F.
Right arrow Articles by Sevoian, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Hamdy, F.
Right arrow Articles by Sevoian, M.
Agricola
Right arrow Articles by Hamdy, F.
Right arrow Articles by Sevoian, M.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1970 September; 20(3): 356-361
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Complement Fixation Reaction for the Diagnosis of Type II Avian (Marek's) Leukosis 1

Farouk Hamdy and Martin Sevoian

Department of Veterinary and Animal Science, University of Massachusetts, Amherst, Massachusetts 01002

ABSTRACT

The present study was designed to find a complement fixation (CF) reaction for the diagnosis of type II lymphoid leukosis, to learn some of the characteristics of the CF antigen, and to investigate the development of CF antibody response to this infection. JM virus-specific antigen was demonstrated in tumorous chicken tissue, in JM virus-infected chick embryo material, in JM virus-infected chicken kidney, and in duck embryo fibroblast tissue culture by using JM virus-immune rabbit serum. This CF antigen did not show cross-reactivity with Rous sarcoma virus or with RIF-type viruses. It was partially heat-labile. The CF activity was restored at —70 C for 10 months and was resistant to intermittent freeze-thaw treatment. The CF antigen may be denatured by ethyl alcohol, but no significant deleterious effects were noted after ether or chloroform treatment. JM virus-specific CF antibody could not be demonstrated by the direct complement dilution method or by the indirect or inhibition form of the CF test in infected or immunized chicken sera.

FOOTNOTES

1 Portion of M.S. Thesis by F.H. Contribution of the Massachusetts Agricultural Experiment Station.

Appl Environ Microbiol. 1970 September; 20(3): 356-361
Copyright © 1970 American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1970 by the American Society for Microbiology. All rights reserved.