This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tippett, L. O. Articles by Robb, C. A. Search for Related Content PubMed Articles by Tippett, L. O. Articles by Robb, C. A. Agricola Articles by Tippett, L. O. Articles by Robb, C. A.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1970 September; 20(3): 342-345
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Modification of the Microtiter Technique for Antimicrobial Drug Susceptibility Testing by Incorporation of Indicators

Alcon Laboratories, Inc., Fort Worth, Texas 76101

ABSTRACT

A pH indicator and dextrose were incorporated into growth media as a modification of microbial microtiter methods for determining the minimal inhibitory concentration of antimicrobial drugs. This modified method was tested to evaluate the ease of reading end points by changes in the indicator color. Application of the procedure to two media, three indicators, and eight species of bacteria indicated that definitive end points could be reached as a result of indicator color change caused by acid production during bacterial growth. This method is accurate and reproducible. It is a modification which eliminates a need for plating and facilitates the reading of minimal inhibitory concentration end points.