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Appl Environ Microbiol. 1970 September; 20(3): 293-297
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Effect of Storage of Mueller-Hinton Agar Plates on Zone Sizes for Antimicrobial Testing

William Pepper Laboratory, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104

ABSTRACT

The length of time Mueller-Hinton agar plates can be stored at 4 C without affecting the size of zones of inhibition in susceptibility testing by the Bauer-Kirby method was studied. It was found that these plates can be stored for 3 weeks at 4 C without an appreciable affect on zone sizes. Storage of plates in sealed plastic bags did not alter the results significantly. The findings indicate that commercially prepared Mueller-Hinton agar plates, which may be several days old when received at the laboratory, are suitable for use in routine susceptibility tests by the Bauer-Kirby method.