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Appl Environ Microbiol. 1970 August; 20(2): 273-275
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Rapid Detection of Sublethally Impaired Cells of Enterobacteriaceae in Dried Foods

Central Institute for Nutrition Research TNO, Zeist, The Netherlands, and San Marcos University, Lima, Peru

ABSTRACT

Samples of dried foods, feeds, and drugs were examined for Enterobacteriaceae by using buffered glucose-Brilliant Green-bile broth as the final enrichment medium and deep tubes of violet-red-bile-glucose-agar for confirmation. The pre-enrichment treatments used for the resuscitation of sublethally impaired cells were overnight incubation in lactose broth versus incubation for 1 to 6 hr at room temperature in shallow layers of tryptone soya peptone broth. The latter restoration treatment appeared to be significantly more productive.