This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lee, S. H. S. Articles by Rozee, K. R. Search for Related Content PubMed Articles by Lee, S. H. S. Articles by Rozee, K. R. Agricola Articles by Lee, S. H. S. Articles by Rozee, K. R.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1970 July; 20(1): 11-15
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Variation of Interferon Production During the Cell Cycle

Department of Microbiology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada

ABSTRACT

The capacity of cells to produce interferon has been found to depend on the phase in the cell cycle at which virus infection took place. Monolayer cultures of L cells were synchronized by the double thymidine-block method. Such synchronously growing cultures were used to study the ability of cells to produce interferon when they were infected with ultraviolet-inactivated Newcastle disease virus (UV-NDV) at different phases of the cell cycle. In all instances, interferon was detected early and reached a maximum at about 16 hr after infection. However, the levels of interferon found in medium of cultures infected at early post-deoxyribonucleic acid (DNA) synthetic (G2) and to some extent at late G2 phases of the cell cycle were comparatively lower than those found in cultures infected at the early DNA synthetic (S) phase. There appeared also in these infected growing cultures a transient period when interferon production was apparently delayed. This period corresponded interestingly with the time of mitotic burst. Infection of thymidine- or 1-ß-D-arabino-furanosylcytosine-inhibited cultures with UV-NDV also led to similar interferon response as that observed in growing cultures infected at early S. However, no transient delay of interferon production was demonstrated in these cultures.

¹ Presently on leave at the Department of Medical Microbiology, The University of Liverpool, Liverpool 3, England.