This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mizrahi, A. Articles by Moore, G. E. Search for Related Content PubMed Articles by Mizrahi, A. Articles by Moore, G. E. Agricola Articles by Mizrahi, A. Articles by Moore, G. E.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1970 June; 19(6): 906-910
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Partial Substitution of Serum in Hematopoietic Cell Line Media by Synthetic Polymers

Public Health Research Group, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York 14203

ABSTRACT

Several synthetic polymers (hydroxyethyl starch, sodium carboxymethyl cellulose, polyvinylpyrrolidone) markedly improved the growth of three human lymphocyte cell lines [Roswell Park Memorial Institute (RPMI) 1348, 1788, and 8098]. Growth was stimulated when each of these polymers was added to RPMI 1640 medium supplemented with only 2% fetal bovine serum. Dextran T-40, T-70, and T-110 varied in their effect on the growth of these cell lines. Dextran T-250 and Haemaccel did not improve cell yields when partially substituted for the serum. The successful partial substitution of polymers for serum was specific for individual cell lines.

¹ Postdoctoral research fellowship from the Israel Institute for Biological Research, Ness-Ziona, Israel