This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Stim, T. B. Search for Related Content PubMed Articles by Stim, T. B. Agricola Articles by Stim, T. B.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1970 May; 19(5): 751-756
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Dengue Virus Plaque Development in Simian Cell Systems

I. Factors Influencing Virus Adsorption and Variables in the Agar Overlay Medium

Yale Arbovirus Research Unit, Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06510

ABSTRACT

Chemical and physical variables influencing the plaquing of all dengue serotypes in two simian cell systems were studied. Calf serum in the nutrient overlay may be replaced by mouse ascitic fluid or bovine plasma albumin when employing the rhesus monkey kidney LLC-MK₂ cell system for plaquing all dengue serotypes. Doubling the serum concentration in the overlay had little effect in modifying dengue types 1, 2, 3, and 4 plaque titers. Newborn agamma, 4-week-old and 8-week-old calf serum gave comparable titers with all dengue virus serotypes. Dengue virus titers, plaque size, and development time were unaffected by sodium bicarbonate concentrations ranging from 1.1 to 4.4 mg/ml of overlay. A twofold increase (0.00332 g%) in the amount of either autoclaved or filtered-sterilized neutral red reduced the dengue 2 virus titer as much as 2.2 logs. An increased Mg⁺⁺ and decreased Ca⁺⁺ concentration in the overlay medium increased the efficiency of the plaquing system.