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Appl Environ Microbiol. 1970 May; 19(5): 718-720
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Production of Chlorflavonin, an Antifungal Metabolite of Aspergillus candidus

Beecham Research Laboratories, Brockham Park, Betchworth, Surrey, England

ABSTRACT

Production of chlorflavonin, a new antifungal antibiotic, by strains of Aspergillus candidus is described. Two wild strains of the fungus had distinctly different chlorflavonin-producing capabilities. One strain produced 25 µg of chlorflavonin per ml per 4 to 5 days in a pilot scale fermentor with stirring, using a medium containing corn steep liquor and glucose. Production of antibiotic was favored by high rates of agitation-aeration. Crude chlorflavonin was extracted from the whole brew with a hydrocarbon solvent and then purified by recrystallization from benzene and petroleum ether. The overall yield from fermentation brew to pure product was 50%.

¹ Present address: The Lord Rank Research Centre, High Wycombe, Bucks, England.