This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Goldstein, M. A. Articles by Orr, H. C. Search for Related Content PubMed Articles by Goldstein, M. A. Articles by Orr, H. C. Agricola Articles by Goldstein, M. A. Articles by Orr, H. C.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1970 April; 19(4): 580-582
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Evaluation of Three Cell Culture Systems as Substrates for Influenza Virus Assay

Laboratory of Virology and Rickettsiology, National Institutes of Health, Bethesda, Maryland 20014

ABSTRACT

The propagation of various influenza virus strains in primary rhesus monkey kidney (RMK), primary hamster kidney (HK), and the MDCK-USD canine kidney (CK) cell cultures was compared. Virus-infected cultures were examined for cytopathic effect (CPE) and by performing the hemadsorption (HAd) technique. The highest HAd titers were found most often in HK, followed by RMK and CK. However, the CK cell line provided the best substrate for detecting CPE. Although influenza B strains tended to grow to higher titers, these strains produced less CPE than did the A strains.