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Appl Environ Microbiol. 1970 March; 19(3): 505-511
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Dehydrogenase Patterns in the Study of Bacteroidaceae¹

Department of Microbiology, School of Medicine, University of Missouri, Columbia, Missouri 65201

ABSTRACT

Enzyme patterns were obtained by starch-gel electrophoresis of cell-free extracts from organisms in the family Bacteroidaceae. Esterases, phosphatases, and lactic and succinic dehydrogenases were detected but offered no possibility for classification purposes. Alpha-glycerophosphate dehydrogenase and 6-phosphogluconate dehydrogenase were not detected. Zymograms of malic, glutamic, and glucose-6-phosphate dehydrogenases separated Bacteroides species from Sphaerophorus and Fusobacterium species. Malic dehydrogenase and glucose-6-phosphate dehydrogenase were found in Bacteroides but not Sphaerophorus and Fusobacterium. These dehydrogenase zymograms placed three gram-negative, nonsporeforming anaerobic rod isolates with the Bacteroides species. A close correlation was found between the classification of Bacteroidaceae by zymogram analysis and a numerical taxonomy scheme previously published from this laboratory.

² Present address: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, N.Y. 10461.

¹ This is a portion of a dissertation submitted by Kenneth C. Keudell to the faculty of the University of Missouri Graduate School in partial fulfillment of the requirements for the Ph.D. degree in Microbiology, August 1969. This work was presented in part at the 69th Annual Meeting of the American Society for Microbiology, Miami Beach, Fla., 4-9 May, 1969.