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Appl Environ Microbiol. 1970 February; 19(2): 287-289
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Separation of Treponema pallidum from Tissue Debris Through Continuous-Particle Electrophoresis

John D. Schmale, Douglas S. Kellogg Jr., Curtis E. Miller1, Patricia Schammel1 and James D. Thayer2

Venereal Disease Research Laboratory, National Communicable Disease Center, Public Health Service, Atlanta, Georgia 30333

ABSTRACT

Treponema pallidum can only be cultured in living animal tissue, such as rabbit testes. However, the extract of these organisms from the testicular material leaves the T. pallidum contaminated with tissue debris. This paper describes the separation of T. pallidum from the debris by continuous-particle electrophoresis. The importance of equilibration time before electrophoresis is discussed.

FOOTNOTES

1 Present address: Beckman Instruments, Inc., Fullerton, Calif. 92634.

2 Deceased 18 July 1968.

Appl Environ Microbiol. 1970 February; 19(2): 287-289
Copyright © 1970 American Society for Microbiology. All Rights Reserved.





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