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Appl Environ Microbiol. 1970 February; 19(2): 278-280
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Selective Medium for Moraxella nonliquefaciens

Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands

ABSTRACT

A medium is described which selectively inhibits most bacteria from the normal flora of the conjunctiva, the lid-margin, and the upper respiratory tract but permits growth of Moraxella nonliquefaciens, M. liquefaciens, and the less fastidious strains of M. lacunata. The selective conditions are obtained with thiostrepton, tyrothricin, and sodium nalidixinate as inhibitors. Incubation under increased CO₂ pressure increased the number of primary isolations. M. nonliquefaciens was isolated on the selective medium twice as many times as on other media.