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Appl Environ Microbiol. 1970 January; 19(1): 53-59
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Potentially Infectious Agents Associated with Shearling Bedpads

I. Effect of Laundering with Detergent-Disinfectant Combinations on Polio and Vaccinia Viruses

Virus Division and Cell Biology Divisions, Southern Research Institute, Birmingham, Alabama 35205
Eastern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture, Philadelphia, Pennsylvania 19118

ABSTRACT

Glutaraldehyde-tanned woolskin pads which are used for the prevention of decubitus ulcers in bed patients were experimentally contaminated with polio or vaccinia viruses. Two methods of exposure, direct contact and aerosol, were used in separate experiments. Attempts were made to remove or inactivate these virus contaminants by laundering the woolskins in a quaternary ammonium disinfectant, a phenolic disinfectant, or alkalinized glutaraldehyde, in combination with an anionic detergent or a nonionic detergent. The effect of a commercial detergent-sanitizer was also studied. The virus titers were significantly reduced in all experiments, but only laundering in glutaraldehyde in combination with either detergent lowered the vaccinia virus titers to below detectable limits. High concentrations of glutaraldehyde altered the texture of the wool and leather apparently by precipitating a component of the detergent onto the fibers. In all the poliovirus experiments, the virus was still detectable on either or both the wool and the leather of the pads after laundering. The rinse water from each experiment was tested for the presence of virus. No vaccinia virus was recovered, but poliovirus was demonstrated in titers up to 10³ cell culture 50% infectious doses.

¹ Present address: ICN Nucleic Acid Research Institute, Irvine, Calif. 92664.