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Appl Environ Microbiol. 1970 January; 19(1): 5-10
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Acid-Precipitated M Protein Compared with a Column-Eluted M Protein Preparation from Type 12, Group A Streptococci

National Communicable Disease Center, Streptococcal Bacteriology Unit, Atlanta, Georgia 30333

ABSTRACT

An M protein preparation of group A streptococci, precipitated with 0.03 M sodium acetate buffer (pH 4.0) was compared with a column-eluted M protein preparation. Absorption spectra and methyl pentose content were similar in both preparations. Acrylamide gel electrophoresis patterns were different. Gel diffusion demonstrated two lines of fusion in the preparations. More antigens could be demonstrated in both preparations by using immunoelectrophoresis. Neither the pH 4 precipitate nor the column-eluted preparation appeared to be a pure M protein preparation.

¹ Present address: Stanford Medical Center, Department of Medicine, Palo Alto, Calif. 94303.