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Appl Environ Microbiol. 1970 January; 19(1): 134-137
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Differentiation of Serratia from Enterobacter on the Basis of Nucleoside Phosphotransferase Production

Department of Laboratory Medicine, University of Minnesota, Minneapolis, Minnesota 55455

ABSTRACT

Precoated cellulose thin-layer chromatograms were used to detect the production of guanosine 5'-phosphate from guanosine and p-nitrophenylphosphate by whole-cell preparations. One-hundred per cent of Serratia (163 strains) and 84% of E. liquefaciens (15 of 18 strains) produced the nucleotide. All other Enterobacter (23 strains), Klebsiella (10 strains), and E. coli (10 strains) were negative for the production of this nucleotide. The entire test procedure could be carried out in 4 hr. It is proposed that E. liquefaciens is more closely related to Serratia than Enterobacter and that reclassification of these organisms should be investigated.

¹ Present address: Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455.