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Appl Environ Microbiol. 1968 September; 16(9): 1348-1351
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Survey of Microorganisms for the Production of Extracellular Phytase

Growth Sciences Center, International Minerals and Chemical Corporation, Libertyville, Illinois 60048

ABSTRACT

A culture enrichment technique was used to isolate phytase-producing microorganisms. Also, microorganisms from various culture collections were tested for their phytase-producing ability. A number of the Aspergillus niger group produced extracellular phytase which dephosphorylated calcium phytate in acidic solution. A soil isolate, A. ficuum NRRL 3135, produced the most active phytase in a cornstarch-based medium. Production of phytase was strongly repressed by inorganic phosphates and required a high carbon to phosphorus ratio in the medium.

¹ Present address: 2921 Glenwood, Billings, Mont. 59102.