This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mann, J. M. Articles by Rheins, M. S. Search for Related Content PubMed Articles by Mann, J. M. Articles by Rheins, M. S. Agricola Articles by Mann, J. M. Articles by Rheins, M. S.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1968 September; 16(9): 1343-1347
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Hemagglutinating Property of Haemophilus aegyptius

Department of Microbial and Cellular Biology, The Ohio State University, Columbus, Ohio 43210

ABSTRACT

Extracts possessing the capacity to hemagglutinate normal human erythrocytes were recovered from Haemophilus aegyptius by treatment with either diethylene glycol or acetone. Antisera prepared against these extracts or the unextracted bacterial cell inhibited hemagglutination by homologous and heterologous antigens. Microgel diffusions indicated the presence of identical components in each extract as expressed by lines of identity between antisera to each fraction. The hemagglutinin was identified as a lipopolysaccharide, 42% lipid and 57% carbohydrate. The determination of 6% phosphorus in the lipid fraction identified it as containing phospholipid.

¹ Present address: Eli Lilly & Co., Indianapolis, Ind. 46206.